Genetic Engineering
Honors Biochemistry II Define these Words
Agarose Gel
Antibiotic Resistance
Agrobacterium
Base pairs (bp)
Cloning
Dideoxy
DNA ligase
E. coli
Fingerprinting
Gel Electrophoresis
Genetic Markers
Molecular Biology
Origin Site (ORI)
Palindrome
Plasmid
Polymerase Chain Reaction (PCR)
Promotor
Recombinant DNA
Restriction Analysis
Restriction Enzymes
Restriction Fragment Length Polymorphism (RFLP)
Sanger Method
Sequencing
Southern Blot Analysis
Sticky ends
Vectors

1. Describe what restriction enzymes recognize and how they cut the DNA.  Why are they so important for the genetic engineer? Where are they from and what was their purpose there?
2. Explain the process required to put a piece of DNA into a plasmid, to put the plasmid into a vector and then make sure the plasmid is present in the vector.
3. Why do all plasmids have an ORI site but not every one has a promotor site?
4. Explain the process of gel electrophoresis.
5. You have been hired as deputy district attorney and must conduct a trial that requires either a RFLP analysis or a PCR.  What conditions would dictate which procedure(s) to use.
6. Describe the process of sequencing using the Sanger method. Be sure to explain how and why the process works.
7. Why do research scientists do genetic engineering?  How is it different for scientists that work for a pharmaceutical company?
8. What is and how do you produce a library or lawn?
9. What is the human genome project and why is biotechnology important for the project?
10. Who are Watson, Crick, Sanger, Gilbert, and LeRoy Hood?  What is the National Institutes of Health and the Department of Energy?  Why are they important for genetic engineering?
11. What are five ethical problems associated with genetic engineering?  After you list the problems be sure to present both pro and anti arguments.